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Objective To analyze ex vivo samples of colorectal cancers by Fourier transform infrared spectrometry and magnetic resonance spectrometry,and to explore the feasibility to diagnose the tumor by using the methods in clinic.Methods From March 2007 to April 2008,fresh samples colorectal mucosa and carcinoma were obtained from 47 patients.The regimens were examined pathologically and then analyzed by Fourier transform infrared spectrometry and magnetic resonance spectrometry.The accuracy of the spectrometrical results was determined by comparing with the pathological results.Results The accuracy of the Fourier transform infrared spectrometry and magnetic resonance spectrometry was 94.7%(89/94)and 97.8%(45/46),respectively,while the sensitivity was 93.6%(44/47)and 100%(23/23),specificity was 95.7%(45/47)and 95.7%(22/23),false positive rate was 4.3%(2/47)and 4.3%(1/23),false negative rate was 6.4%(3/47)and 0%(0/23),positive prognostic value was 95.7%(44/46)and 95.8%(23/24),and the negative prognostic value was 93.8%(45/48)and 100%(22/22).ConclusionsBenign and malignant colorectal tissues can be identified quickly and accurately by Fourier transform infrared spectrometry and magnetic resonance spectrometry.The methods,which are minimally invasive,could be a potential diagnosing tool for colorectal cancer at an early stage.
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AIM:To observe the expression of calcium-sensing receptor(CaSR) of rat cardiomyocytes in anoxia-reoxygenation(A/R) injury and CaSR-induced changes of intracellular calcium;involvement of CaSR in apoptosis and relevant signaling transduction pathway.METHODS:The A/R injury was remodeled in vitro;CaSR,caspase 3 and caspase 9 were deteced by Western blotting.LSCM was used to observe changes of intracellular calcium during reperfusion with or without CaSR agonist.Morphological changes in different groups were observed by light microscopes.Apoptotic cells were measured by TUNEL assay.RESULTS:By LSCM,it was found that the intracellular calcium was significantly increased during reperfusion both in A/R and activator group.Severe injury was found by HE staining in the above two groups,the number of apoptotic cells was significantly increased according to TUNEL assay.The expression of CaSR,caspase 3 and caspase 9 was significantly increased in A/R group and activator group compared with control.CONCLUSION:CaSR is involved in intracellular calcium overload in A/R cardiomyocyte,which can accelerate apoptosis during A/R.
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Aim To observe the protective effect of quercetin on rat cardiomyocyte apoptosis induced by adriamycin and explore its possible mechanism.Methods Cultured neonatal rat cardiomyocytes were randomly divided into six groups:normal control group, adriamycin group,quercetin control group, adriamycin+quercetin(25,50,100 ?mol?L-1)groups. The activity of LDH was detected by chromatometry, the cardiomyocyte viability was measured by MTT, the ultrastructure of cardiomyocyte was observed by electron microscope, the expression of protein Bcl-2 and Bax was analyzed by immunocytochemical, and the mRNA and protein of caspase-3 were detected by RT-PCR and Western blot respectively.Results Compared with the control group, the activity of LDH was increased but the viability of cardiomyocyte was decreased; the expression of Bax and caspase-3 was up-regulated while Bcl-2 was down-regulated in ADR group.Compared with ADR group, the above changes were lightened in adriamycin+quercetin groups. But the quercetin control group, in which cultured myocardial cells only exposed to quercetin without ADR, had no obvious changes.Conclusions Quercetin significantly inhibits the apoptosis induced by ADR in the cultured myocardial cells. Its mechanism is involved in the apoptosis-related pathways, including caspase-3, Bax and Bcl-2.